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At this time 1.five mL of acetic anhydride was included and permitted to react at place temperature overnight. Just after elimination of the solvent, the residue was dissolved in 100 mL of chloroform and washed with dilute HCl, saturated NaHCO3 then dried above Na2SO4. The product or service, diphenyl N
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a could be the acid dissociation regular. pH-rate profiles that showed the lack of catalytic activity at substantial and lower values of pH were being in shape to equation four, where K
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Incubation from the apo-enzyme with 1 equal of Zn2+ for 30 minutes completely restored catalytic action. The titration of apo-enzyme with various amounts of ZnCl2 is proven in Figure one. These final results display that NagA from E. coli
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